| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schultz, Claire L (15)
|
WIWYNS |
24.02 |
25.52 |
1.50 |
| 2 |
Mayer, Sara M (16)
|
WIWYNS |
24.44 |
25.63 |
1.19 |
| 3 |
Schultz, Grace M (15)
|
WIWYNS |
24.33 |
25.77 |
1.44 |
| 4 |
Theiler, Kylee S (17)
|
WIWYNS |
23.90 |
25.86 |
1.96 |
| 5 |
Hoff, Sydney D (16)
|
WISPDY |
25.56 |
25.93 |
0.37 |
| 6 |
Gargulak, Sidney F (17)
|
WISPDY |
|
26.06 |
- |
| 7 |
Breitbach, Alaina M (17)
|
WIWYNS |
24.77 |
26.32 |
1.55 |
| 8 |
Blennert, Evelyn R (15)
|
WIWYNS |
25.23 |
26.62 |
1.39 |
| 9 |
Clifford, Emma F (16)
|
WIWYNS |
25.29 |
27.07 |
1.78 |
| 10 |
Swiecki, Brooke (15)
|
WISPDY |
26.88 |
27.15 |
0.27 |
| 11 |
Bissen, Phoebe (15)
|
WIDCY |
25.76 |
27.52 |
1.76 |
| 12 |
Kracht, Tara E (15)
|
WIMYST |
26.74 |
27.69 |
0.95 |
| 13 |
Donahue, Courtney A (15)
|
WIMYST |
26.44 |
27.88 |
1.44 |
| 14 |
Nguyen, Amber T (17)
|
WILAXY |
27.32 |
27.98 |
0.66 |
| 15 |
Johnston, Anna E (15)
|
WISPDY |
27.83 |
28.58 |
0.75 |
| 16 |
Stimac, Paige (15)
|
WISWC |
28.98 |
28.67 |
-0.31 |
| 17 |
Placeway, Brianna (15)
|
WISPDY |
27.59 |
28.69 |
1.10 |
| 18 |
Engelbright, Rachel L (15)
|
WIWYNS |
30.46 |
29.33 |
-1.13 |
| 19 |
Herheim, Anika (16)
|
WISPDY |
29.97 |
30.20 |
0.23 |
| 20 |
Geesey, Aurora (15)
|
WISPDY |
31.55 |
32.74 |
1.19 |
|
Gilbertson, Kennedy E (18)
|
WISPDY |
24.13 |
23.87 |
-0.26 |